Elucidation of regulatory network for isoprenoid biosynthesis using correlation coefficients from microarray expression data

DOI
  • Sano Ryosuke
    The NEDO Team of Applied Plant Genomics, Kazusa DNA Res. Inst.
  • Ogata Yoshiyuki
    The NEDO Team of Applied Plant Genomics, Kazusa DNA Res. Inst.
  • Sakurai Nozomu
    The NEDO Team of Applied Plant Genomics, Kazusa DNA Res. Inst.
  • Aoki Koh
    The NEDO Team of Applied Plant Genomics, Kazusa DNA Res. Inst.
  • Suzuki Hideyuki
    The NEDO Team of Applied Plant Genomics, Kazusa DNA Res. Inst.
  • Saito Kazuki
    The NEDO Team of Applied Plant Genomics, Kazusa DNA Res. Inst. Graduate School of Pharmaceutical Sciences, Chiba Univ.
  • Shibata Daisuke
    The NEDO Team of Applied Plant Genomics, Kazusa DNA Res. Inst.

Bibliographic Information

Other Title
  • イソプレノイド合成経路の制御系の解明にむけた発現相関のネットーワーク解析

Abstract

We investigated the regulatory mechanisms of isoprenoid biosynthesis, especially with reference to cis-prenyltransferase (cPT) which is involved in rubber synthesis. By analyzing co-expression of isoprenoid biosynthesis genes using the correlation coefficients calculated from 771 AtGenExpress data, some gene networks are inferred. 1) Genes in methylerythritol phosphate (MEP) pathway were closely connected, whereas those of mevalonate (MVA) pathway were only clustered when using the categorized array data from tissues. 2) Phosphomevalonate kinase gene was constantly isolated from the cluster of mevalonate pathway genes owing to its constitutive high expression. 3) Using the tissue-categorized array data each cPT gene was not connected with other cPT genes or the isoprenoid biosynthesis genes, but connected with some transcription factor genes. <br>For candidate transcription factors for cPT, generation of transgenic lines with overexpression or RNAi construct is in progress.

Journal

Details 詳細情報について

  • CRID
    1390001205628891776
  • NII Article ID
    130006989229
  • DOI
    10.14841/jspp.2006.0.109.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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