Production of transgenic soybean plants overexpressing plant cysteine proteinase inhibitors to confer insect resistance

DOI

Bibliographic Information

Other Title
  • 植物シスタチン遺伝子導入による耐虫性ダイズの作出

Abstract

Bean bug,Riptortus clavatus, causes a serious damage of soybean production. The insect pest utilizes cysteine proteinases for protein digestion. In contrast, plants contain proteinous inhibitors, cystatins, against endogenous and exogenous cysteine proteinases including insect digestive proteases. In this research, we produced transgenic soybeans overexpressing plant cystatins to confer the insect resistance. We used three plant cystatins, OC1, mCC1 and JTC, from rice, corn, and Job's tears, respectively. Each of the cystatin genes was connected with a seed specific promoter derived from soybean 7S globulin gene or CaMV35S promoter, and nos-terminator, and the resultant cassette was inserted between the green fluorescence protein gene and the hygromycin resistance gene in a pUHG plasmid vector. The plasmids were introduced into soybean embryogenic cells by particle bombardment. Several transgenic plants were obteined by introduction of these constructs and we are now developing fixed lines for transgenes to analyze the resistance to R. clavatus.

Journal

Details 詳細情報について

  • CRID
    1390001205629011328
  • NII Article ID
    130006989378
  • DOI
    10.14841/jspp.2005.0.491.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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