Preparation of UDP-Galacturonic Acid Using UDP-Sugar Pyrophosphorylase
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- Ohashi Takao
- Graduate School of Science, Osaka Univ.
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- Cramer Nicolai
- Graduate School of Science, Osaka Univ. Inst. Org. Chem., Univ. Stuttgart
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- Ishimizu Takeshi
- Graduate School of Science, Osaka Univ.
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- Hase Sumihiro
- Graduate School of Science, Osaka Univ.
Bibliographic Information
- Other Title
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- UDP糖ピロホスホリラーゼを用いたUDP-ガラクツロン酸の合成
Abstract
UDP-D-galacturonic acid (UDP-GalUA) is a donor of galacturonic acid in pectin. This compound is not commercially available, and its preparative method with high efficiency is needed. UDP-GalUA pyrophosphorylase involved UDP-GalUA synthesis has been neither purified nor cloned. The UDP-sugar pyrophosphorylase from Pisum sativum possesses a broad substrate specificity. Here, we investigated UDP-GalUA pyrophosphorylase activity of this enzyme and applied it to the preparation of UDP-GalUA.<br>The UDP-sugar pyrophosphorylase catalyzed the formation of UDP-GalUA and pyrophosphate from GalUA 1-phosphate and UTP, showing that this enzyme has UDP-GalUA pyrophosphorylase activity. The enzyme had optimum pH of 6 and the Km values for GalUA 1-phosphate, UTP, UDP-GalUA, and pyrophosphate were 2.3 mM, 1.2 mM, 0.70 mM, and 1.3 mM, respectively. In the presence of inorganic pyrophosphatase, the enzyme produced UDP-GalUA in 91 % yield on a preparative scale. Thus this UDP-sugar pyrophosphorylase is useful for the highly efficient production of UDP-GalUA.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2006 (0), 185-185, 2006
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390001205629252608
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- NII Article ID
- 130006989674
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed