Isolation and characterization of <I>Arabidopsis</I> mutants with altered response to sulfur

DOI

Bibliographic Information

Other Title
  • 硫黄応答が変化したシロイヌナズナ変異株の単離と解析

Abstract

The β subunit gene of soybean β-conglicinin of has been shown to be upregulated by sulfur deficiency at the level of transcription. Insertion of the 235 bp-sulfur responsive promoter region of this gene into cauliflower mosaic virus 35S promoter enabled us to observe response to sulfur in non-seed tissues (Awazuhara et al. 2002). In transgenic Arabidopsis NOB plants carrying this chimeric promoter fused with GFP gene, GFP fluorescence was increased by sulfur deficiency (Ohkama et al. 2002). We screened mutants with altered GFP fluorescence from M2 plants of EMS-mutagenized NOB plants. <br>nbm1-1 (NOB mutant) shows higher GFP fluorescence than parental NOB plants irrespective of sulfur condition. Expression of Sultr2;2, APR1. SAT1, endogenous sulfur-responsive genes, were also upregulated. genetic mapping located the nbm1-1 locus in upper arm of Chromosome 5. Awazuhara et al. (2002) Plant Sci. 163: 75-82, Ohkama et al. (2002) Plant Cell Physiol. 43: 1266-1275

Journal

Details 詳細情報について

  • CRID
    1390001205629621504
  • NII Article ID
    130006990094
  • DOI
    10.14841/jspp.2003.0.695.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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