Isolation and characterization of <I>Arabidopsis</I> mutants with altered response to sulfur
-
- Ohkama Naoko
- Graduate School of Agricultural and Life Sciences, Univ. of Tokyo
-
- Hayashi Hiroaki
- Graduate School of Agricultural and Life Sciences, Univ. of Tokyo
-
- Yoneyama Tadakatsu
- Graduate School of Agricultural and Life Sciences, Univ. of Tokyo
-
- Naito Satoshi
- Graduate School of Agriculture, Hokkaido Univ
-
- Fujiwara Toru
- Graduate School of Agricultural and Life Sciences, Univ. of Tokyo
Bibliographic Information
- Other Title
-
- 硫黄応答が変化したシロイヌナズナ変異株の単離と解析
Abstract
The β subunit gene of soybean β-conglicinin of has been shown to be upregulated by sulfur deficiency at the level of transcription. Insertion of the 235 bp-sulfur responsive promoter region of this gene into cauliflower mosaic virus 35S promoter enabled us to observe response to sulfur in non-seed tissues (Awazuhara et al. 2002). In transgenic Arabidopsis NOB plants carrying this chimeric promoter fused with GFP gene, GFP fluorescence was increased by sulfur deficiency (Ohkama et al. 2002). We screened mutants with altered GFP fluorescence from M2 plants of EMS-mutagenized NOB plants. <br>nbm1-1 (NOB mutant) shows higher GFP fluorescence than parental NOB plants irrespective of sulfur condition. Expression of Sultr2;2, APR1. SAT1, endogenous sulfur-responsive genes, were also upregulated. genetic mapping located the nbm1-1 locus in upper arm of Chromosome 5. Awazuhara et al. (2002) Plant Sci. 163: 75-82, Ohkama et al. (2002) Plant Cell Physiol. 43: 1266-1275
Journal
-
- Plant and Cell Physiology Supplement
-
Plant and Cell Physiology Supplement 2003 (0), 695-695, 2003
The Japanese Society of Plant Physiologists
- Tweet
Details 詳細情報について
-
- CRID
- 1390001205629621504
-
- NII Article ID
- 130006990094
-
- Data Source
-
- JaLC
- CiNii Articles
-
- Abstract License Flag
- Disallowed