Chemical Genetic Analysis on Arabdiopsis Seed Germination
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- Tsuchiya Yuichiro
- Department of Cell and Systems Biology, University of Toronto Dormancy and Adaptation Research Team, RIKEN Plant Science Center
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- Kamiya Yuji
- Dormancy and Adaptation Research Team, RIKEN Plant Science Center
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- Nambara Eiji
- Dormancy and Adaptation Research Team, RIKEN Plant Science Center
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- Cutler Sean
- Department of Cell and Systems Biology, University of Toronto
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- McCourt Peter
- Department of Cell and Systems Biology, University of Toronto
Bibliographic Information
- Other Title
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- ケミカルジェネティクスを用いたシロイヌナズナ種子発芽のメカニズムの解析
Abstract
Although genetic analysis has identified genes involved in seed germination many components still remain to be discovered. We have investigated seed germination of Arabidopsis using chemical genetics. As with mutational analysis, the phenotype caused by small molecule are used as a starting point to identify genes in a particular biological process.<br> From 10,000 non-annotated chemical library, 5 compounds (E compounds) were identified which inhibit cotyledon greening and expansion. Interestingly ABA or GA signaling mutants showed altered response to E compounds. Consistent with this, genetic screening for E compounds resistant mutants identified new alleles of ABA and GA response mutants. Surprisingly, suppressor screens also identified new alleles of elongated hypocotyl1 locus (hy1). Although hy1 is known photomorphogenesis mutant it is also allele of genome uncoupled2 locus which is involved in plastid retrograde signal. In this presentation, we will discuss possible involvement of E compounds on hormone, light and plastid retrograde signaling.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2007 (0), 040-040, 2007
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390001205629881344
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- NII Article ID
- 130006990316
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed