Functional Analyses of Cell Wall Formation-Related Genes Estimated from <I>Arabidopsis thaliana</I> Gene Coexpression Profiles

DOI

Bibliographic Information

Other Title
  • シロイヌナズナ遺伝子共発現相関解析から予測された細胞壁形成関連遺伝子の機能解明

Abstract

Cellulose, a major component of the cell walls, is the most abundant biopolymer in plants and serves many uses as industrial materials. However, the cellulose synthesis is so complicated that the mechanisms have been poorly unraveled. By analyzing comprehensive gene coexpression profiles based on publicly available microarray datasets, we identified several transcription factor (TF) genes that were coexpressed with cell wall formation genes.<br>We experimentally examined functions of the TFs using Arabidopsis cultured cell T87. Overexpression of one of the TF genes, tightly coexpressed with genes involved in secondary cell wall formation, caused phenotype change, which was confirmed by Fourier transform infrared spectrophotometer analysis. RNAi-induction of other TFs that coexpressed with genes involved in primary cell wall formation using glucocorticoid-inducible transcription system revealed lethal phenotypes when grown on dexamethasone. With a focus on these TFs, we will also report microarray analyses and metabolomic analyses using mass spectrometry.

Journal

Details 詳細情報について

  • CRID
    1390001205630405632
  • NII Article ID
    130006991104
  • DOI
    10.14841/jspp.2007.0.210.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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