Characterization of Geranyl Diphosphate Synthase from <I>Lithospermum erythrorhizon</I>
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- Miyawaki Tatsuya
- Research Institute for Sustainable Humanosphere, Kyoto Univ.
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- Sasaki Kanako
- Research Institute for Sustainable Humanosphere, Kyoto Univ.
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- Yamamoto Kyoko
- Research Institute for Sustainable Humanosphere, Kyoto Univ.
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- Ohara Kazuaki
- Research Institute for Sustainable Humanosphere, Kyoto Univ.
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- Sakurai Nozomu
- Kazusa DNA Research Institute
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- Suzuki Hideyuki
- Kazusa DNA Research Institute
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- Shibata Daisuke
- Kazusa DNA Research Institute
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- Yazaki Kazufumi
- Research Institute for Sustainable Humanosphere, Kyoto Univ.
Bibliographic Information
- Other Title
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- ムラサキ由来ゲラニルジリン酸合成酵素の機能解析
Abstract
Lithospermum erythrorhizon produces shikonin derivatives, naphthoquinone-type secondary metabolites, in the roots. Shikonin is formed by the coupling of two key intermediates, geranyl diphosphate (GPP) derived from mevalonate pathway and p-hydroxybenzonate via shikimate pathway. It is proved that GPP is provided from mevalonate pathway and the GPP synthase is localized in cytosol as a sole exception in higher plants characterized thus far. From EST information we cloned a homolog of FPP synthase (LeFPPS) as a candidate of GPP synthase of cytosolic localization. Recombinant LeFPPS purified via His-tag demonstrated FPPS activity from GPP and IPP as substrates but not GPPS and FPPS activity from DMAPP and IPP. However, the correlated expression of LeFPPS with shikonin accumulation suggested that the in vivo function of LeFPPS is GPPS. Indeed, the addition of cell-free-extract of L.erythrorhizon converted the FPPS activity to strong GPPS activity, suggesting LeFPPS functions as a catalytic subunit of GPPS in vivo.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2007 (0), 823-823, 2007
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390001205630421888
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- NII Article ID
- 130006991123
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed