遺伝子発現のリズムを測る--生物発光リアルタイム・モニタリング・システムの開発と、概日リズム研究への応用

DOI
  • Okamoto Kazuhisa
    Center for Gene Research, Nagoya University JST, Nagoya University
  • Onai Kiyoshi
    Center for Gene Research, Nagoya University JST, Nagoya University
  • Ishiura Masahiro
    Center for Gene Research, Nagoya University JST, Nagoya University Division of Biological Science, Graduate School of Science, Nagoya University

書誌事項

タイトル別名
  • Development Of A High-throughput Real-time Bioluminescence-monitoring System

抄録

Real-time monitoring of gene expression using a bioluminescence reporter strain is one of the most powerful methods for the analysis of gene expression in living cells with a high sensitivity, a broad dynamic range, and a high time-resolution. The method allows easy isolation of a large number of mutants affecting the expressions of any genes in the genome. However, there were many problems in previous systems. We have developed a high-throughput real-time bioluminescence monitoring system, which consists of two types of high-throughput bioluminescence monitoring apparatus and the bioluminescence-analyzing program RAP. The system enables the automatic monitoring and analysis of bioluminescence from 4,800 individual samples in one assay under uniform light conditions.<br> Here, we will introduce the real-time bioluminescence monitoring system and the cloning and analysis the circadian clock genes in cyanobacteria and Arabidopsis.

収録刊行物

詳細情報 詳細情報について

  • CRID
    1390001205630939648
  • NII論文ID
    130006991895
  • DOI
    10.14841/jspp.2007.0.s001.0
  • データソース種別
    • JaLC
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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