遺伝子発現のリズムを測る--生物発光リアルタイム・モニタリング・システムの開発と、概日リズム研究への応用
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- Okamoto Kazuhisa
- Center for Gene Research, Nagoya University JST, Nagoya University
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- Onai Kiyoshi
- Center for Gene Research, Nagoya University JST, Nagoya University
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- Ishiura Masahiro
- Center for Gene Research, Nagoya University JST, Nagoya University Division of Biological Science, Graduate School of Science, Nagoya University
書誌事項
- タイトル別名
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- Development Of A High-throughput Real-time Bioluminescence-monitoring System
抄録
Real-time monitoring of gene expression using a bioluminescence reporter strain is one of the most powerful methods for the analysis of gene expression in living cells with a high sensitivity, a broad dynamic range, and a high time-resolution. The method allows easy isolation of a large number of mutants affecting the expressions of any genes in the genome. However, there were many problems in previous systems. We have developed a high-throughput real-time bioluminescence monitoring system, which consists of two types of high-throughput bioluminescence monitoring apparatus and the bioluminescence-analyzing program RAP. The system enables the automatic monitoring and analysis of bioluminescence from 4,800 individual samples in one assay under uniform light conditions.<br> Here, we will introduce the real-time bioluminescence monitoring system and the cloning and analysis the circadian clock genes in cyanobacteria and Arabidopsis.
収録刊行物
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- 日本植物生理学会年会およびシンポジウム 講演要旨集
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日本植物生理学会年会およびシンポジウム 講演要旨集 2007 (0), S001-S001, 2007
日本植物生理学会
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詳細情報 詳細情報について
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- CRID
- 1390001205630939648
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- NII論文ID
- 130006991895
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可