Clarification of Metabolic Disorders and Identification of Marker Metabolites by FT-ICR MS

DOI

Bibliographic Information

Other Title
  • FT-ICR MSメタボロミクス解析による代謝変調の高速解析と変調マーカー分子同定

Abstract

We established a metabolic profiling analysis system using FT-ICR MS (Fourier Transform Ion Cyclotron Resonance Mass Spectrometry) in combination with a data analysis tool (Dmass) and a metabolite-species database (KNApSAcK). The entire scheme was applied for the clarification of metabolic disorders in Arabidopsis seedlings treated with herbicides of different mode of actions. Arabidopsis extracts were directly infused into the ESI ion source on an FT-ICR MS system. Acquired metabolic data consisting of m/z values with ion intensity values were subjected to a principal component analysis. Metabolic disorders induced by the herbicide treatments were clearly differentiated from the herbicide-free metabolome. From each herbicide treatment, m/z values of ions representing the metabolic disorder were identified, and metabolite candidates search were then performed through a KNApSAcK database search. The database search and MSMS analyses manifested a dose dependent accumulation of shikimic acid 3-phosphate and several flavonoid glycosides in the specific herbicides treatment.

Journal

Details 詳細情報について

  • CRID
    1390001205630958464
  • NII Article ID
    130006991922
  • DOI
    10.14841/jspp.2006.0.888.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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