Clarification of Metabolic Disorders and Identification of Marker Metabolites by FT-ICR MS
-
- Oikawa Akira
- Graduate School of Life and Environmental Sciences, Osaka Prefecture Univ. Research Association for Biotechnology
-
- Ogura Tomonori
- Graduate School of Life and Environmental Sciences, Osaka Prefecture Univ.
-
- Kimura Atsuko
- Graduate School of Life and Environmental Sciences, Osaka Prefecture Univ.
-
- Nakamura Yukiko
- Department of Bioinformatics and Genomics, NAIST
-
- Sakurai Nozomu
- Kazusa DNA Res. Inst.
-
- Suzuki Hideyuki
- Kazusa DNA Res. Inst.
-
- Saito Kazuki
- Graduate School of Pharmaceutical Sciences, Chiba Univ.
-
- Shibata Daisuke
- Kazusa DNA Res. Inst.
-
- Kanaya Shigehiko
- Department of Bioinformatics and Genomics, NAIST
-
- Ohta Daisaku
- Graduate School of Life and Environmental Sciences, Osaka Prefecture Univ.
Bibliographic Information
- Other Title
-
- FT-ICR MSメタボロミクス解析による代謝変調の高速解析と変調マーカー分子同定
Abstract
We established a metabolic profiling analysis system using FT-ICR MS (Fourier Transform Ion Cyclotron Resonance Mass Spectrometry) in combination with a data analysis tool (Dmass) and a metabolite-species database (KNApSAcK). The entire scheme was applied for the clarification of metabolic disorders in Arabidopsis seedlings treated with herbicides of different mode of actions. Arabidopsis extracts were directly infused into the ESI ion source on an FT-ICR MS system. Acquired metabolic data consisting of m/z values with ion intensity values were subjected to a principal component analysis. Metabolic disorders induced by the herbicide treatments were clearly differentiated from the herbicide-free metabolome. From each herbicide treatment, m/z values of ions representing the metabolic disorder were identified, and metabolite candidates search were then performed through a KNApSAcK database search. The database search and MSMS analyses manifested a dose dependent accumulation of shikimic acid 3-phosphate and several flavonoid glycosides in the specific herbicides treatment.
Journal
-
- Plant and Cell Physiology Supplement
-
Plant and Cell Physiology Supplement 2006 (0), 888-888, 2006
The Japanese Society of Plant Physiologists
- Tweet
Details 詳細情報について
-
- CRID
- 1390001205630958464
-
- NII Article ID
- 130006991922
-
- Data Source
-
- JaLC
- CiNii Articles
-
- Abstract License Flag
- Disallowed