The development of an efficient method for isolating RNA from blueberry leaves
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- Fuse Takuichi
- Miyazaki Prefectural Industrial Support Foundation
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- Nishiwaki Aya
- Fac. of Agri., Miyazaki Univ.
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- Kunitake Hisato
- Fac. of Agri., Miyazaki Univ.
Bibliographic Information
- Other Title
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- ブルーベリー葉における効率的なRNA抽出法の開発
Description
Blueberry leaves are rich in antioxidants, and are expected to be useful as a material of functional foods. We have made DNA markers related to polyphenol synthesis genes by cDNA subtraction, and have tried to select cultivars including many polyphenols using DNA markers. It is difficult to isolate RNA from blueberry leaves because the leaves include many polyphenols. We established an efficient method for isolating RNA from blueberry leaves by a modification of the cetyl trimethyl ammonium bromide (CTAB) method. We isolated RNA from blueberry leaves using eight different buffers: borate, HEPES, MES, MOPS, PIPES, TES, Tricine and Tris. We obtained about 80 μg of RNA from 1g of blueberry leaves with a widely used Tris buffer. We obtained more than 200μg of RNA from 1 g of blueberry leaves with a HEPES or a MOPS buffer. The amount of RNA was sufficient for cDNA synthesis. We succeeded at performing cDNA synthesis and increasing the cDNA using polymerase chain reactions. We constructed a number of cDNA subtraction libraries, and have recently isolated cDNA fragments related to polyphenol synthesis genes from these libraries.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2010 (0), 0719-0719, 2010
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390001205631074304
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- NII Article ID
- 130006992087
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed