An application of the Micro-Tom mutant resource: Aiming at the clarification of the biosynthetic mechanism of tomatine

DOI

Bibliographic Information

Other Title
  • マイクロトム変異体リソースの活用：トマチンの生合成機構の解明に向けて

Description

It has been generally accepted that the biosynthetic pathway of phytosteroid is only via cycloartenol. However, recently, we identified lanosterol synthase gene (LAS1) from Arabidopsis and discovered that a part of phytosteroid is biosynthesized via lanosterol by the tracer experiment of labeled mevalonate (MVL). Then, why does plant have the biosynthetic pathway of phytosteroid via lanosterol? Since LAS1 expression is induced by methyl jasmonate treatment, the metabolites of lanosterol may be plant defense related compounds. Tomatine, steroidal alkaroid, which seem to be related to defense response, included in Tomato. Although it is thought that tomatine is biosynthesized from phytosterol, the biosynthetic mechanisms are poorly understood. We undertook the present study to identify the genes for tomatine biosynthesis using Micro-Tom, which is a model plant of Tomato. By the tracer experiment of MVL, it was suggested that biosynthetic pathway via lanosterol exists in the plant. Now, we are screening EMS mutation lines for tomatine biosynthesis deficient mutants by TLC analysis. In this symposium, we discuss the possibility of biosynthetic studies using Micro-Tom.

Journal

Details 詳細情報について

  • CRID
    1390001205631460480
  • NII Article ID
    130006992674
  • DOI
    10.14841/jspp.2010.0.s0037.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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