Metabolite Annotations Based on the Integration of Mass Spectral Information.
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- Iijima Yoko
- Kazusa DNA Res. Inst.
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- Nakamura Yukiko
- Kazusa DNA Res. Inst. Ehime Woman's college
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- Sakurai Nozomu
- Kazusa DNA Res. Inst.
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- Ogata Yoshiyuki
- Kazusa DNA Res. Inst.
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- Tanaka Kenichi
- Nara Institute of Science and Technology
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- Suda Kunihiro
- Kazusa DNA Res. Inst.
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- Suzuki Tatsuya
- Kazusa DNA Res. Inst.
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- Suzuki Hideyuki
- Kazusa DNA Res. Inst.
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- Okazaki Koei
- Kazusa DNA Res. Inst.
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- Kanaya Shigehiko
- Nara Institute of Science and Technology
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- Aoki Koh
- Kazusa DNA Res. Inst.
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- Shibata Daisuke
- Kazusa DNA Res. Inst.
Bibliographic Information
- Other Title
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- 質量分析情報による代謝物アノテーションデータベースの作成と応用
Abstract
We previously proposed a procedure of metabolite annotation to identify mass signals representing metabolites by analyzing LC-FTICR-MS data. Here, we applied the metabolite annotation procedure to Micro-Tom fruit, and constructed the metabolite annotation database, which facilitates a comprehensive characterization of tomato fruit metabolites. Analysis of methanol extract of the fruits at different ripening stages enabled us to provide annotations to 869 metabolites in total, of which 494 appeared to be novel. The number of metabolites was larger in the peel than the flesh at all stages, and increased with ripening. Modifications such as glucosylation and acylation frequently occurred in fruit metabolites. We annotated 70 flavonoids and 93 glycoalkaloids, and confirmed that they undergo various modifications during ripening. The metabolite annotation procedure was further applied to comparative analyses of metabolites in fruits of mutant lines. This work was partly supported by Research and Development Program for New Bio-Industry Initiatives.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2008 (0), 0133-0133, 2008
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390001205631806592
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- NII Article ID
- 130006993183
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed