Comprehensive analysis of transcriptome and metabolome using transgenic plants overexpressing stress-inducible transcription factors DREB1A and DREB2A
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- Maruyama Kyonoshin
- Biol. Resources Div. JIRCAS
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- Takeda Migiwa
- Kazusa DNA Res. Inst.
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- Urano Kaoru
- PSC RIKEN
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- Sakuma Yoh
- Biol. Resources Div. JIRCAS
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- Kidokoro Satoshi
- Univ. Tokyo., Grad. Sch. Agr. Life Sci.
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- Suzuki Hideyuki
- Kazusa DNA Res. Inst.
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- Saito Kazuki
- Kazusa DNA Res. Inst. Chiba Univ., Grad. Sch. Pharm. Sci.
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- Shibata Daisuke
- Kazusa DNA Res. Inst.
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- Shinozaki Kazuo
- CREST, JST PSC RIKEN
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- Yamaguchi-Shinozaki Kazuko
- Biol. Resources Div. JIRCAS CREST, JST Univ. Tokyo., Grad. Sch. Agr. Life Sci.
Bibliographic Information
- Other Title
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- 低温および乾燥環境下におけるシロイヌナズナのトランスクリプト-ムおよびメタボロ-ム解析
Abstract
Plants respond and adapt to cold and drought stresses with physiological and developmental changes. These stresses induce some kinds of genes which function in stress tolerance. Overexpression of DREB1A and/or DREB2A in transgenic plants activated the expression of many stress-inducible genes and resulted in improved tolerance to freezing and/or drought. To identify cold- and/or drought-stress-inducible metabolic enzyme genes and downstream genes of DREB1A and/or DREB2A, we performed 22k oligarray analysis. We identified known and new cold- and/or drought-stress-inducible metabolic enzyme genes. Some kinds of metabolic enzyme genes were included in downstream genes of DREB1A and/or DREB2A. To monitor metabolites in the cold and/or drought treated plants and transgenic plants overexpressing DREB1A and/or DREB2A, we assessed metabolites on a large scale by using GC/MS, LC/MS and CE/MS. Some kinds of sugers, amino acids and flavonoides were found to increase in cold and/or drought acclimated plants and transgenic plants.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2006 (0), 801-801, 2006
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390001205632135552
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- NII Article ID
- 130006993668
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed