A comprehensive metabolite analysis of Arabidopsis plant overexpressing a vacuolar membrane transporter candidate gene

DOI

Bibliographic Information

Other Title
  • 液胞膜トランスポーター候補遺伝子過剰発現シロイヌナズナ植物体の代謝物解析

Abstract

Vacuole of plant cells contributes to the storage of various types of metabolites. However, mechanisms of metabolic regulation between cytosol and vacuole have not been clarified yet. To elucidate mechanisms controlling substrate trafficking, we have been analyzing functions of vacuolar transporter candidate genes. One of our targets is a gene encoding vacuolar MATE efflux family protein. We have demonstrated the effect of overexpression of this gene on the metabolite accumulation pattern by using Arabidopsis suspension cultured cells and isolated vacuoles. In this study, we analyzed metabolite accumulation profile of Arabidopsis plant overexpressing this gene. We focused on the analysis of semi-polar metabolites accumulated in the rosette leaves using LC-FTICR-MS. Analysis of data obtained by ESI ionization mode demonstrated that accumulation of flavonol and indol compounds increased in the overexpressor, whereas accumulation of tryptophane and sulfur-containing metabolites decreased. Putative function of this MATE efflux family protein gene is discussed in terms of vacuolar transport. This work was partly supported by CREST, JST.

Journal

Details 詳細情報について

  • CRID
    1390001205632273664
  • NII Article ID
    130006993878
  • DOI
    10.14841/jspp.2010.0.0976.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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