Title Interaction analysis between COP9 signalosome subunit1 and novel transcription factors.

DOI

Bibliographic Information

Other Title
  • COP9シグナロソーム と相互作用する転写因子の機能解析

Description

COP9 signalosome (CSN) is a nuclear complex regulating signal transduction in plants and animals. To dissect the function of CSN, we focus on the function of CSN1 subunit. The N-terminal region of CSN1 (CSN1N) possessed a transcriptional repression activity in mammals, and was essential for survival in plants. To understand this mechanism, Yeast Two-Hybrid method was utilized to isolate proteins interacting with CSN1N in Arabidopsis. Here, we isolated a transcription factor containing a predicted structural domain composed of three helixes and two loops. In Arabidopsis, transcription factors with this conserved domain form a novel family. This conservation was preserved in plants, but not in mammals, which suggests that these genes may encode proteins with plant-specific functions. In pull-down assays, this transcription factor, and two others from the family, was shown to directly bind CSN1N. Here, we characterize these transcriptional factors, and discuss their role in CSN regulation.

Journal

Details 詳細情報について

  • CRID
    1390001205632358272
  • NII Article ID
    130006994013
  • DOI
    10.14841/jspp.2009.0.0843.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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