Is bicarbonate a ligand to the oxygen evolving Mn cluster?: Analyses by FTIR spectroscopy
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- Aoyama Chika
- Inst. of Materials Sci., Univ. of Tsukuba
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- Suzuki Hiroyuki
- Inst. of Materials Sci., Univ. of Tsukuba
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- Sugiura Miwa
- Dept. Appl. Bio. Chem.,Osaka Pref. Univ.
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- Noguchi Takumi
- Inst. of Materials Sci., Univ. of Tsukuba
Bibliographic Information
- Other Title
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- 炭酸水素イオンは酸素発生マンガンクラスターの配位子か?:赤外分光法による解析
Abstract
Bicarbonate is known to be a ligand to the non-heme iron located between QA and QB in PSII. It has also been proposed that bicarbonate is a ligand to the Mn cluster and controls O2-evolving reactions. However, details of its structural relationship remain unclarified. In this study, structural coupling of bicarbonate with the Mn cluster was studied using Fourier transform infrared (FTIR) spectroscopy. Flash-induced FTIR difference spectra of S-state cycle were recorded using PSII in the presence of H12CO3- or H13CO3-. In 12C -minus-13C double difference spectra, bicarbonate bands were observed at the 1st flash but no bands were detected at the 2nd-4th flashes. These bicarbonate bands were identical to those in the non-heme iron spectrum measured with Mn-depleted PSII. Thus, no bicarbonate band arising from the S-state cycle was detected in FTIR spectra. This result strongly suggests that bicarbonate is not a ligand to the Mn cluster.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2007 (0), 235-235, 2007
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390001205632489216
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- NII Article ID
- 130006994210
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed