Gene Function Analysis Using FT-ICR/MS Metabolomics with the van Krevelen Diagram
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- Kai Kosuke
- Research Association for Biotechnology Graduate School of Life and Environmental Sciences, Osaka Prefecture University
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- Minami Kayo
- Graduate School of Life and Environmental Sciences, Osaka Prefecture University
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- Tsuyuguchi Keitaro
- School of Life and Environmental Sciences, Osaka Prefecture University
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- Kitano Shinya
- Graduate School of Life and Environmental Sciences, Osaka Prefecture University
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- Takahashi Hiroki
- Graduate School of Information Science, Nara Institute of Science and Technology
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- Shojo Masayuki
- Graduate School of Information Science, Nara Institute of Science and Technology
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- Sakurai Nozomu
- Kazusa DNA Research Institute
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- Suzuki Hideyuki
- Kazusa DNA Research Institute
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- Shibata Daisuke
- Kazusa DNA Research Institute
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- Kanaya Shigehiko
- Graduate School of Information Science, Nara Institute of Science and Technology
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- Ohta Daisaku
- Graduate School of Life and Environmental Sciences, Osaka Prefecture University
Bibliographic Information
- Other Title
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- van Krevelen diagram を用いたメタボローム解析による遺伝子機能の探索
Abstract
Accurate mass measurement using Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR/MS) allows prediction of elemental compositions of each analyte. We have powered our FT-ICR/MS-based metabolomics scheme by implementing an analytical function for reconstituting metabolic activities on the van Krevelen diagram, which is constructed by plotting atomic ratios of each elemental composition. In this report, we discuss the possibility of this analytical tool for gene function analysis. Practically, metabolome data from wild-type plants and T-DNA insertion mutant lines were converted to elemental compositions with ion intensity information, and were superimposed on the van Krevelen diagram. Analysis of a cytochrome P450 and an acyltransferase genes using this scheme allowed us to speculate metabolic activities related to these genes of interest. These novel enzyme functions were elucidated by enzyme assay with recombinant proteins, demonstrating the effectiveness of our new approach.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2009 (0), 0314-0314, 2009
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390001205632563712
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- NII Article ID
- 130006994319
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed