Comparative Metabolic Profiling of Ripening-mutant Tomato Fruits.
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- Iijima Yoko
- Kazusa DNA Reserch Institute
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- Sakurai Nozomu
- Kazusa DNA Reserch Institute
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- Suzuki Hideyuki
- Kazusa DNA Reserch Institute
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- Aoki Koh
- Kazusa DNA Reserch Institute
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- Shibata Daisuke
- Kazusa DNA Reserch Institute
Bibliographic Information
- Other Title
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- ライプニング変異体トマト果実の代謝プロファイル解析
Abstract
Tomato is a climacteric fruit whose ripening is induced by ethylene. Remarkable metabolic changes in tomato fruits occur during ripening, however, it is unclear which metabolites are affected by ethylene, and how ethylene controls metabolic pathways of these metabolites. To confirm metabolites responding to ethylene comprehensively, we investigated metabolic profiling of ripening-mutant tomato fruits. Mutant tomato fruits inhibiting ethylene production or perception (nor, rin and Nr) and their wild type (cv. Rutgers) were grown in the same condition and harvested at mature green stage and red-ripe stage of wild type. Primary metabolites were analyzed by GC-MS after TMS-derivatization, and approximately 100 metabolites were identified and compared. Secondary metabolites were analyzed by UPLC-TOFMS and their metabolic profiles were compared. The metabolite annotation data, consisting of about 800 metabolites, were used for prediction of structures of secondary metabolites. These comprehensive analyses enabled to discriminate the metabolite which is affected by ethylene.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2009 (0), 0312-0312, 2009
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390001205632565760
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- NII Article ID
- 130006994322
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed