Comparison of leaf carbonyl profiles among ecotypes of Arabidopsis thaliana
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- Mano Jun'ichi
- Science Research Center, Yamaguchi Univ.
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- Tokushige Kenji
- Faculty of Agriculture, Yamaguchi Univ.
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- Khorobrykh Sergey
- Science Research Center, Yamaguchi Univ.
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- Iijima Yoko
- Kazusa DNA Institute
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- Shibata Daisuke
- Kazusa DNA Institute
Bibliographic Information
- Other Title
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- シロイヌナズナ葉に含まれるカルボニル化合物組成のエコタイプ間比較
Abstract
Lipid peroxide-derived carbonyls (aldehydes and ketones) are reactive species. Like reactive oxygen species, they work as signal molecules at low concentrations and damage various cellular components at high concentrations. In order to elucidate the regulation and physiological effects of carbonyls in leaves, we here established a comprehensive analysis of carbonyls. Leaves of Arabidopsis thaliana were extracted with acetonitrile. The extract was derivatized with 2,4-dinitrophenylhydrazine, and separated on a reversed-phase HPLC column. More than 50 species of carbonyls were detected, among which aldehydes and ketones of carbon chain length 3-10 with or without C=C double bond, were identified by comparison of their retention time with standard compounds. Three ecotypes Col-0, Nos, and Ler, showed similar profiles of carbonyls except that Col-0 showed lower 3Z-hexenal and 2E-hexenal levels, corresponding well with the lack of hydroperoxide lyase (HPL) activity in Col-0. This result imply the HPL-independent production of aldehydes in leaves.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2007 (0), 347-347, 2007
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390001205632889472
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- NII Article ID
- 130006994811
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed