Comparison of leaf carbonyl profiles among ecotypes of Arabidopsis thaliana

DOI

Bibliographic Information

Other Title
  • シロイヌナズナ葉に含まれるカルボニル化合物組成のエコタイプ間比較

Abstract

Lipid peroxide-derived carbonyls (aldehydes and ketones) are reactive species. Like reactive oxygen species, they work as signal molecules at low concentrations and damage various cellular components at high concentrations. In order to elucidate the regulation and physiological effects of carbonyls in leaves, we here established a comprehensive analysis of carbonyls. Leaves of Arabidopsis thaliana were extracted with acetonitrile. The extract was derivatized with 2,4-dinitrophenylhydrazine, and separated on a reversed-phase HPLC column. More than 50 species of carbonyls were detected, among which aldehydes and ketones of carbon chain length 3-10 with or without C=C double bond, were identified by comparison of their retention time with standard compounds. Three ecotypes Col-0, Nos, and Ler, showed similar profiles of carbonyls except that Col-0 showed lower 3Z-hexenal and 2E-hexenal levels, corresponding well with the lack of hydroperoxide lyase (HPL) activity in Col-0. This result imply the HPL-independent production of aldehydes in leaves.

Journal

Details 詳細情報について

  • CRID
    1390001205632889472
  • NII Article ID
    130006994811
  • DOI
    10.14841/jspp.2007.0.347.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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