cDNA cloning and intracellular localization of tobacco type 2 prolyl 4-hydroxylases
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- Moriguchi Ryo
- Graduate School of Agriculture, Kyushu University
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- Suyama Akiko
- Graduate School of Agriculture, Kyushu University
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- Osawa Yukiko
- RIKEN Plant Science Center
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- Matsuoka Ken
- Graduate School of Agriculture, Kyushu University RIKEN Plant Science Center
Bibliographic Information
- Other Title
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- タバコの2型プロリン水酸化酵素遺伝子のクローニングと局在解析
Abstract
The first step of plant-specific protein O-glycosylation is the hydroxylation of proline residue catalyzed by prolyl 4-hydroxylases (P4Hs). To investigate this modification, we have been analyzing P4Hs in tobacco. This time we cloned three full-length cDNAs for type2 P4H (NtP4H2.1, NtP4H2.2, NtP4H2.3). All the encoded proteins contain N-terminal signal peptide, catalytic domain and C-terminal Tox1 domain, the function of which is unknown. Membrane fractionation analysis using a specific antibody for NtP4H2.2 revealed NtP4H2.2 distributes in fractions containing Golgi apparatus. NtP4H2.2 fused with GFP co-localized with a cis-Golgi marker protein in tobacco BY-2 cells. Almost all NtP4H2.2 was recovered in sedimentable fraction after the sonication of microsome. These results indicate type2 P4H is a membrane protein localized predominantly in the Golgi apparatus. We are currently investigating the function of the Tox1 domain and results of this analysis will be presented.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2009 (0), 0791-0791, 2009
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390001205633022208
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- NII Article ID
- 130006994998
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed