cDNA cloning and intracellular localization of tobacco type 2 prolyl 4-hydroxylases

DOI

Bibliographic Information

Other Title
  • タバコの2型プロリン水酸化酵素遺伝子のクローニングと局在解析

Abstract

The first step of plant-specific protein O-glycosylation is the hydroxylation of proline residue catalyzed by prolyl 4-hydroxylases (P4Hs). To investigate this modification, we have been analyzing P4Hs in tobacco. This time we cloned three full-length cDNAs for type2 P4H (NtP4H2.1, NtP4H2.2, NtP4H2.3). All the encoded proteins contain N-terminal signal peptide, catalytic domain and C-terminal Tox1 domain, the function of which is unknown. Membrane fractionation analysis using a specific antibody for NtP4H2.2 revealed NtP4H2.2 distributes in fractions containing Golgi apparatus. NtP4H2.2 fused with GFP co-localized with a cis-Golgi marker protein in tobacco BY-2 cells. Almost all NtP4H2.2 was recovered in sedimentable fraction after the sonication of microsome. These results indicate type2 P4H is a membrane protein localized predominantly in the Golgi apparatus. We are currently investigating the function of the Tox1 domain and results of this analysis will be presented.

Journal

Details 詳細情報について

  • CRID
    1390001205633022208
  • NII Article ID
    130006994998
  • DOI
    10.14841/jspp.2009.0.0791.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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