Increased production level of hyaluronan in transgenic tobacco cultured cells

DOI

Bibliographic Information

Other Title
  • タバコ培養細胞におけるヒアルロン酸生産能の向上

Abstract

Hyaluronan is a linear polysaccharide composed of alternating β1,4-glucuronic acid and β1,3-N-acetylglucosamine moieties. Recently, hyaluronan has extended the applications in various fields including cosmetics, medicine, health-care food, etc. Commercially available hyaluronan is isolated from rooster comb or produced with microbial fermentation. We have introduced the chlorella virus hyaluronan synthase (cvHAS) gene into tobacco BY-2 cells or tobacco plants via Agrobacterium-mediated transformation and confirmed the production of hyaluronan in the transgenic plant cells. Production level of hyaluronan in the transgenic BY-2 cells was increased by coexpressing UDP-D-glucose dehydroganase(Ugd) and L-glutamine D-fructose-6-phosphate amidotransferase (GFAT) with cvHAS.

Journal

Details 詳細情報について

  • CRID
    1390001205633052416
  • NII Article ID
    130006995041
  • DOI
    10.14841/jspp.2009.0.0801.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

Report a problem

Back to top