Increased production level of hyaluronan in transgenic tobacco cultured cells
-
- Shibatani Shigeo
- TOYOBO Co., Ltd.
-
- Hamada Emi
- TOYOBO Co., Ltd.
-
- Nagaya Shingo
- NAIST
-
- Shibata Daisuke
- Kazusa
-
- Shinmyo Atsuhiko
- NAIST
-
- Kato Ko
- NAIST
Bibliographic Information
- Other Title
-
- タバコ培養細胞におけるヒアルロン酸生産能の向上
Abstract
Hyaluronan is a linear polysaccharide composed of alternating β1,4-glucuronic acid and β1,3-N-acetylglucosamine moieties. Recently, hyaluronan has extended the applications in various fields including cosmetics, medicine, health-care food, etc. Commercially available hyaluronan is isolated from rooster comb or produced with microbial fermentation. We have introduced the chlorella virus hyaluronan synthase (cvHAS) gene into tobacco BY-2 cells or tobacco plants via Agrobacterium-mediated transformation and confirmed the production of hyaluronan in the transgenic plant cells. Production level of hyaluronan in the transgenic BY-2 cells was increased by coexpressing UDP-D-glucose dehydroganase(Ugd) and L-glutamine D-fructose-6-phosphate amidotransferase (GFAT) with cvHAS.
Journal
-
- Plant and Cell Physiology Supplement
-
Plant and Cell Physiology Supplement 2009 (0), 0801-0801, 2009
The Japanese Society of Plant Physiologists
- Tweet
Details 詳細情報について
-
- CRID
- 1390001205633052416
-
- NII Article ID
- 130006995041
-
- Data Source
-
- JaLC
- CiNii Articles
-
- Abstract License Flag
- Disallowed