ChIP-based Analysis of Transcriptional Regulation in <I>Arabidopsis thaliana</I> Chloroplasts

DOI
  • Hanaoka Mitsumasa
    Fac. of Horticul., Chiba Univ. Inst. of Mol. Cell. Biosci., The Univ. of Tokyo
  • Kato Maiko
    Fac. of Horticul., Chiba Univ.
  • Azuma Miyuki
    Inst. of Mol. Cell. Biosci., The Univ. of Tokyo
  • Tanaka Kan
    Fac. of Horticul., Chiba Univ. Inst. of Mol. Cell. Biosci., The Univ. of Tokyo

Bibliographic Information

Other Title
  • クロマチン免疫沈降法を利用したシロイヌナズナ葉緑体転写制御系の解析

Abstract

Chloroplasts have their own DNA and gene expression systems. In order to study transcriptional regulation, biochemical and genetic approaches have been historically used. However, biochemical approaches does not usually demonstrate functions under physiological conditions, and genetic approaches may include some indirect effects, which make it difficult to understand specific regulation by the transcription factors. Chromatin immunoprecipitation (ChIP) is a powerful and useful tool that can obtain information for binding sites for transcription factors, and directly detect dynamic changes of their interaction patterns in vivo.<br>To further understand transcriptional regulation in Arabidopsis thaliana chloroplasts, we here developed ChIP-based method, and analyzed binding pattern of SIG5, a stress-induced chloroplast sigma factor. We found SIG5 specifically binds to target promoters such as psbA or psbD BLRP, and this binding depends on several kinds of stress conditions. This result suggests that ChIP analysis is useful to understand transcriptional regulation of chloroplast genes, which can overcome several problems from traditional methods.

Journal

Details 詳細情報について

  • CRID
    1390001205633063936
  • NII Article ID
    130006995057
  • DOI
    10.14841/jspp.2010.0.0355.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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