Induction by Single Low-dose Irradiations of Acute Transcriptional Alterations in Several Alzheimer's Disease-Related Genes without Significant Late Consequences on Behavioral and Brain Pathological Changes in Mice

DOI
  • WANG Bing
    Research Center for Radiation Protection, National Institute of Radiological Sciences, Chiba, Chiba 263-8555, Japan
  • TANAKA Kaoru
    Research Center for Radiation Protection, National Institute of Radiological Sciences, Chiba, Chiba 263-8555, Japan
  • JI Bin
    Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, Chiba 263-8555, Japan
  • ONO Maiko
    Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, Chiba 263-8555, Japan
  • FANG Yaqun
    Research Center for Charged Particle Therapy, National Institute of Radiological Sciences, Chiba, Chiba 263-8555, Japan
  • NINOMIYA Yasuharu
    Research Center for Radiation Protection, National Institute of Radiological Sciences, Chiba, Chiba 263-8555, Japan
  • MARUYAMA Kouichi
    Research Center for Radiation Protection, National Institute of Radiological Sciences, Chiba, Chiba 263-8555, Japan
  • NAKAJIMA Nakako
    Research Center for Charged Particle Therapy, National Institute of Radiological Sciences, Chiba, Chiba 263-8555, Japan
  • SHANG Yi
    Research Center for Radiation Protection, National Institute of Radiological Sciences, Chiba, Chiba 263-8555, Japan
  • BEGUM Nasrin
    Centre for Nuclear Medicine and Ultrasound, Rajshahi 6000, Bangladesh
  • HIGUCHI Makoto
    Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, Chiba 263-8555, Japan
  • FUJIMORI Akira
    Research Center for Charged Particle Therapy, National Institute of Radiological Sciences, Chiba, Chiba 263-8555, Japan
  • UEHARA Yoshihiko
    Graduate School of Medicine, Tohoku University, Sendai, Sendai 980-8575, Japan
  • NAKAJIMA Tetsuo
    Research Center for Radiation Protection, National Institute of Radiological Sciences, Chiba, Chiba 263-8555, Japan
  • SUHARA Tetsuya
    Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, Chiba 263-8555, Japan
  • ONO Tetsuya
    Graduate School of Medicine, Tohoku University, Sendai, Sendai 980-8575, Japan
  • NENOI Mitsuru
    Research Center for Radiation Protection, National Institute of Radiological Sciences, Chiba, Chiba 263-8555, Japan

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Other Title
  • 低線量単回照射によるアルツハイマー病関連遺伝子の転写レベルの一過性変化は晩発障害として有意な行動異常や脳の病理学的変化を引き起こさない

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Abstract

Alzheimer's disease (AD) is the most common form of dementia while its cause and progression are not well understood. The possible cognitive and behavioral consequences induced by low-dose radiation are of great concern as humans are exposed to ionizing radiations from various sources including medical diagnosis. A recent study in mice reported early transcriptional response in brain to low-dose X-rays (0.1Gy) suggesting alterations of molecular networks and pathways associated with cognitive functions, advanced aging and AD. To investigate the cognitive and behavioral consequences induced by low doses, C57BL/6J mice were whole-body irradiated with either X-rays (0.1Gy) or accelerated carbon ions (0.05 Gy or 0.1 Gy). The hippocampus was collected and the expression of AD-related genes was analyzed. Morris Water maze test was applied to the measurement of the learning ability and memory of the animals. Amyloid imaging with positron emission tomography (PET) were performed to detect the accumulation of fibrillary amyloid beta peptide, and characteristic pathologies of AD were examined with immunohistochemical staining of amyloid precursor protein (APP), amyloid beta peptide, tau and phosphorylated tau. Results showed significant acute transcriptional alterations in several AD-related genes at 4 h after irradiation while no marked changes 1 yr later. No significant difference on learning ability and memory was observed 1 yr after irradiation. Imaging and immunohistochemical staining showed that no change in the accumulation of fibrillar amyloid and the expression of APP, amyloid beta peptide, tau, phosphorylated tau were not detectable in the animals 4 m and 2 yr after irradiation. These findings suggest that low-dose irradiations could induce acute transcriptional alterations in several AD-related genes without significant late consequences on behavioral and AD-related pathological changes in mice.

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