Prospective study on adaptive response induction by heavy-ion radiation in human lymphoblastoid cell lines

DOI

Bibliographic Information

Other Title
  • ヒトリンバ芽球細胞におけるX線及び重粒線による適応応答誘導に関する研究

Abstract

In a variety of in vitro and in vivo models, pre-exposure to low priming dose of ionizing radiations is well known to decrease the biological effects of a subsequent higher challenging dose. However, very few data exist concerning the possibility of inducing this phenomenon, called radiation-induced adaptive response (AR), when using high-LET accelerated heavy-ion radiation. In this study, cultured human lymphoblastoid cells (with different p53 status: TK6, AHH-1, NH32) were exposed to X-rays, carbon-ion and neon-ion radiation at various LETs (ranging from 20 to 150 keV/micrometer). Mutation frequency at HPRT locus, radiation-induced cell death, cell cycle effects after irradiation, as well as estimation of DNA double-strand breaks induction and repair by measuring gamma-H2AX phosphorylation kinetics were compared in primed and unprimed cells. Our results suggest that the biological effects resulting from exposure to priming and challenging radiations vary according to irradiation type and LET. An AR to mutagenic effects of heavy-ion radiation at higher LET was observed when cells were exposed to priming doses of X-rays. The ability of low dose and low dose-rate heavy-ion radiation to trigger an AR was also assessed.

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Details 詳細情報について

  • CRID
    1390001205641467136
  • NII Article ID
    130007000945
  • DOI
    10.11513/jrrsabst.2007.0.69.0
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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