ビタミンB_<12>類縁体のグルタミン酸誘発神経細胞死に対する保護作用

DOI Web Site 参考文献33件

書誌事項

タイトル別名
  • Protective Effects of Vitamin B_<12> Analogs Against Glutamate-induced Neuronal Death
  • ビタミン B12 ルイエンタイ ノ グルタミンサン ユウハツ シンケイ サイボ

この論文をさがす

説明

The effects of methylcobalamin and cyanocobalamin, vitamin B_<12> analogs, on glutamate-induced neuronal death were examined using cortical cultures. Cell viability was markedly reduced by a brief exposure to glutamate followed by 1-hour incubation with glutamate-free medium. Glutamate-induced neurotoxicity was prevented by MK-801, an antagonist of N-methyl-D-aspartate (NMDA) subtype of glutamate receptors. Glutamate neurotoxicity was inhibited by removing extracellular Ca^<2+> during glutamate exposure. Glutamate neurotoxicity was also inhibited by N^ω-nitro-L-arginine, a nitric oxide (NO) synthase inhibitor, and hemoglobin, which traps NO. NO donors such as sodium nitroprusside induced neurotoxicity with the manner similar to that induced by glutamate. These results indicate that delayed neuronal death induced by glutamate is mediated by NO, which is formed by Ca^<2+> influx via NMDA receptors. Glutamate neurotoxicity was significantly ameliorated when the cultures were maintained in the medium containing either methylcobalamin or cyanobalamin. By contrast, acute exposure of cultures to vitamin B_<12> analogs did not affect glutamate neurotoxicity. Glutamate neurotoxicity was reduced by chronic exposure to S-adenosylmethionine, which is formed in the metabolic pathway of methylcobalamin. neurotoxicity induced by sodium nitroprusside was reduced by methylcobalamin and S-adenosylmethionine. These results indicate that chronic exposure to vitamin B_<12> analogs protects cortical neurons against glutamate neurotoxicity by reducing neurotoxic action of NO.

収録刊行物

  • ビタミン

    ビタミン 69 (11), 631-643, 1995

    公益社団法人 日本ビタミン学会

参考文献 (33)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ