書誌事項
- タイトル別名
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- Biochemistry of Selenium Compounds and Pyridoxal 5'-phosphate enzymes
- セレン カゴウブツ ト ピリドキサール 5 リンサン コウソ ノ セイカガク
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説明
Pyridoxal 5'-phosphate-dependent enzymes, selenocysteine lyase (SCL) and cysteine desulfurase, occur in a variety of organisms. SCL catalyzes the decomposition of L-selenocysteine to form L-alanine and an enzyme-bound cysteine perselenide (Cys-Se) intermediate. Cysteine desulfurase catalyzes the same type of reaction as SCL but acts on L-cysteine as a substrate to form an cysteine persulfide (Cys-SH) on the active site residue. We carried out comparative studies between SCL and cysteine desulfurase to investigate functional and mechanistic characteristics of the family of the enzymes. Spectrum analyses showed that L-cysteine enters into the active site pocket of SCL but can not form an external aldimine complex with PLP. The formation of an external aldimine complex between SCL and L-selenocysteine requires the active-site nucleophile Cys375. Thus, Cys375 of SCL plays a central role in the discrimination between selenium and sulfur in a substrate and its analog.
収録刊行物
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- ビタミン
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ビタミン 80 (12), 587-595, 2006
公益社団法人 日本ビタミン学会
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詳細情報 詳細情報について
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- CRID
- 1390001205701067264
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- NII論文ID
- 110006152692
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- NII書誌ID
- AN00207833
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- ISSN
- 2424080X
- 0006386X
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- NDL書誌ID
- 8630553
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDLサーチ
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
