Possibility of application of basic fibroblast growth factor and glycosaminoglycans to bone regeneration therapy

DOI
  • Kannzaki Shin
    Division of Oral and Maxillofacial Reconstructive Surgery, Department of Oral and Maxillofacial Surgery , Kyushu Dental College
  • Kannno Takahiro
    Division of Oral and Maxillofacial Reconstructive Surgery, Department of Oral and Maxillofacial Surgery , Kyushu Dental College
  • Ariyoshi Wataru
    Division of Oral and Maxillofacial Reconstructive Surgery, Department of Oral and Maxillofacial Surgery , Kyushu Dental College
  • Ichimiya Hisashi
    Division of Oral and Maxillofacial Reconstructive Surgery, Department of Oral and Maxillofacial Surgery , Kyushu Dental College
  • Shinnmyouzu Kouhei
    Division of Oral and Maxillofacial Reconstructive Surgery, Department of Oral and Maxillofacial Surgery , Kyushu Dental College
  • Takahashi Tetsu
    Division of Oral and Maxillofacial Reconstructive Surgery, Department of Oral and Maxillofacial Surgery , Kyushu Dental College
  • Nishihara Tatuji
    Division Infections and Molecular Biology, Department Health Promotion, Kyushu Dental College

Bibliographic Information

Other Title
  • 塩基性線維芽細胞増殖因子（bFGF）とグリコサミノグリカン（GAGs）の骨再生療法への応用の可能性

Description

Recent studies have shown that basic fibroblast growth factor (bFGF) promotes the growth of osteoblasts and bone formation. It has been shown that glycosaminoglycans (GAGs) including chondroitin sulfate (CS), promote bone formation and play an important role in control of bone metabolism. However, detailed mechanisms remain unclear. In order to examine the application to the bone regeneration therapy in clinical, the mechanisms of the proliferation and differentiation of the osteoblasts by the combined applycation of bFGF and GAG was examined in in vitro assay system. bFGF, CS, and hyaluronic acid (HA) were added to mouse preosteoblastic MC3T3-E1 cells , and the cells were cultured. The degree of proliferation and the differentiation respectively were evaluated with MTT assay and ALP enzyme activity assay. bFGF upregulated the proliferation of preosteoblasts. CS upregulated the cell proliferation induced by bFGF, and inhibited the attenuation of the ALP activity. On the other hand, HA upregulated cell proliferation of bFGF, almost no change were found on the attenuation of the ALP activity. Our results indicate that the combined application of bFGF and CS may be useful in the bone regeneration therapy.

Journal

Keywords

Details 詳細情報について

  • CRID
    1390001205713344256
  • NII Article ID
    130007022030
  • DOI
    10.11495/jkds.kds65.0.10.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

Report a problem

Back to top