バゾプレッシン-eGFPトランスジェニックラットの下垂体後葉における<I>in vitro</I> eGFP蛍光連続測定の試み
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- 藤原 広明
- 産業医科大学・医・第1生理学
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- Yao Song
- ブリストル大学・医・神経内分泌学
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- Paton Julian
- ブリストル大学・医・生理学
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- 上田 陽一
- 産業医科大学・医・第1生理学
書誌事項
- タイトル別名
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- Development of <I>in vitro</I> eGFP monitoring system in arginine vasopressin-eGFP transgenic rat posterior pituitary.
抄録
We developed enhanced green fluorescent protein (eGFP) monitoring system which is able to measure eGFP that appeared to the living cell by the optical fiber, continuously. This system irradiates the excitation light to the neuron and the nerve ending where eGFP exists and the optical fluorescent signal is converted into the electric signal. In this study, using this system, we continuously measured change of fluorescence in arginine vasopressin (AVP)-eGFP transgenic rat posterior pituitary (PP) with several physiological stimulations. High potassium solution, salusinβ, and galanin-like peptide (GALP) showed a marked decrease of fluorescent signal that suggested AVP secretion from the nerve endings in the PP. These results suggest that this monitoring system has the possibility that AVP secretion can be indirectly monitored, as index the change of fluorescence. [J Physiol Sci. 2007;57 Suppl:S173]
収録刊行物
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- 日本生理学会大会発表要旨集
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日本生理学会大会発表要旨集 2007 (0), 173-173, 2007
一般社団法人 日本生理学会
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詳細情報 詳細情報について
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- CRID
- 1390001205727309440
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- NII論文ID
- 130007038597
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
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