Ultrastructural visualization of the transmembrane nicotinic AChR of frog motor endplate by means of an immunochemical reaction with d-tubocurarine.

DOI
  • Hirai Keiji
    Dept. Autonom. Physiol., Med. Res. Inst., Tokyo Med. Dent. Univ., Tokyo, Japan
  • Katayama Yoshifumi
    Dept. Autonom. Physiol., Med. Res. Inst., Tokyo Med. Dent. Univ., Tokyo, Japan
  • Ichinose Shizuko
    Instrumental Analysis Res. Center, Tokyo Med. Den. Univ., Tokyo, Japan
  • Peltre Gabriel
    Lab. Allergie et Environment, LECA, Ecole Superieure de Physique et Chimie Industrielles, 75005-Paris, France
  • Okada Yumi
    Dept. Autonom. Physiol., Med. Res. Inst., Tokyo Med. Dent. Univ., Tokyo, Japan
  • Tsuji Shigeru
    Dept. Cytology, Univ. P. & M. Curie, 75005-Paris, France

Bibliographic Information

Other Title
  • カエル終板ニコチン性アセチルコリン受容体のクラーレと免疫反応による超微細構造の可視化

Abstract

D-tubocurarine (d-TC) was used to block the synaptic transmission of frog neuromuscular junction. A recently discovered avidity of IgG for d-TC and also for other quaternary ammonium compounds (Tsuji et al 2003) was used to visualize the d-TC bound to the nicotinic acetylcholine receptor (nAChR) by means of an immunochemical reaction (immunoperoxidase). Regularly disposed transmembrane rod-like structures were observed through the postsynaptic membrane and interpreted as a staining of the interior side of the nAChR. Reference: Tsuji, S. et al (2003) Binding avidity of immunoglobulin G for acetylcholine. Biomed Res 24: 217-221 [J Physiol Sci. 2007;57 Suppl:S151]

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Details 詳細情報について

  • CRID
    1390001205728331776
  • NII Article ID
    130005449027
  • DOI
    10.14849/psjproc.2007.0_151_4
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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