The Alteration of Endogeneous Keratin Networks after Transfection of Hair Keratin Genes
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- Niwa Masanao
- Department of Dermatology, Juntendo University School of Medicine
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- Manabe Motomu
- Department of Dermatology, Akita University School of Medicine
Bibliographic Information
- Other Title
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- ヘアーケラチン遺伝子導入による内在性ケラチンネットワークの変化
- ヘアーケラチン イデンシ ドウニュウ ニ ヨル ナイザイセイ ケラチンネットワーク ノ ヘンカ
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Description
To define the interactions reguired for the filament assembly of differentiation-specific keratins, active copies of mouse hair keratin mHa 1 and mHb 4 genes were introduced into a rat kangaroo kidney epitherial cell line (PtK 2) and a rat stratified squamous epitherial cell line (rat epidermal keratinocyte). In PtK2 transient transfectants, when introduced individually or in combination, mHa 1 and mHb 4 formed aggregates of ring-like structures of various sizes at the perinuclear region with no evidence of organization into a keratin network. These aggregates altered the distribution of the endogeneous keratins and vimentin. In most of the cells carrying the ring-like structures of mHa 1 and mHb 4 around their nuclei, the endogenous keratin network collapsed and localized around the nucleus. Furthermore, the densely accumulated endogenous keratin surrounded the ring-like aggregates with partial co-localization. However, when transfected into the rat epidermal keratinocytes, mHa 1 and mHb 4 were able to co-localize with the well-developed cytoskeleton of endogenous keratins. These results showed that, in constrast to keratin pairs K5/K14and K8/K18, the mHa 1/mHb 4 pair is unable to develop an extensive keratin network on its own, and that there are possible defferential abilities among these hair keratins and other keratins to form well-developed cytoplasmic networks.
Journal
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- The Japanese Journal of Dermatology
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The Japanese Journal of Dermatology 111 (2), 139-145, 2001
Japanese Dermatological Association
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Keywords
Details 詳細情報について
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- CRID
- 1390001205739043968
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- NII Article ID
- 10007909129
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- NII Book ID
- AN00196602
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- ISSN
- 13468146
- 0021499X
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- NDL BIB ID
- 025120510
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- CiNii Articles
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- Abstract License Flag
- Disallowed