Characterization of the first hemin-requiring <i>Pseudomonas aeruginosa</i> small-colony variant isolated from the blood of a patient with double pneumonia
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- NAGANO Natsumi
- Department of Clinical Laboratory, Kansai Medical University Medical Center
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- NAKAYA Hideo
- Department of Clinical Laboratory, Kansai Medical University Medical Center
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- NATSUME Seiko
- Department of Clinical Laboratory, Kansai Medical University Medical Center
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- NAGATA Megumi
- Department of Clinical Laboratory, Kansai Medical University Medical Center
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- HONJYO Hideyasu
- Department of Clinical Laboratory, Kansai Medical University Medical Center
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- KAWAHARA Ryuji
- Division of Bacteriology, Osaka Institute of Public Health
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- TAKADA Atsuaki
- Department of Clinical Laboratory, Kansai Medical University Medical Center
Bibliographic Information
- Other Title
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- 血液培養から<i>Pseudomonas aeruginosa</i>のヘミン要求性Small-Colony Variantを検出した1症例
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Abstract
<p>A hemin-requiring Pseudomonas aeruginosa small-colony variant (SCV) was isolated from the blood of a patient with double pneumonia. The isolate was capable of growing on both blood and chocolate agar plates but not on MacConkey agar plates without blood. Furthermore, the isolate failed to grow on triple-sugar iron agars. The microscopic morphology of the isolate was similar to that of Haemophilus species. When examined the auxotrophy using X-factor (hemin)- and V-factor (NAD)-impregnated discs on the Mueller-Hinton agar plate, the isolate grew only around the X-factor (hemin)-impregnated disc. However, when using the VITEK2 system, the isolate was identified as Acinetobacter lwoffii. The isolate was finally identified as P. aeruginosa by 16S rRNA gene sequencing and MALDI-TOF MS analysis. SCV phenotypes are related to persistent and recurrent infections. Moreover, it is difficult to identify SCVs because of their atypical colony morphology and altered biochemical profile. The accurate identification of SCVs is important for effective antimicrobial treatment. To the best of our knowledge, this is the first clinical isolation of a hemin-dependent P. aeruginosa SCV.</p>
Journal
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- Japanese Journal of Medical Technology
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Japanese Journal of Medical Technology 67 (1), 99-104, 2018
Japanese Association of Medical Technologists
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Keywords
Details 詳細情報について
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- CRID
- 1390001205743099136
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- NII Article ID
- 130006321357
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- ISSN
- 21885346
- 09158669
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- Text Lang
- ja
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed