KAMPO MEDICINE “DAIKENCHUTO” PREVENTS THE DECREASE OF INTERSTITIAL CELLS OF CAJAL IN MICE WITH CONSTIPATION INDUCED BY MORPHINE

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  • YOSHIDA Yuri
    Department of Physiology, Showa University School of Medicine Department of Dermatology, Showa University School of Medicine
  • SUNAGAWA Masataka
    Department of Physiology, Showa University School of Medicine
  • KUSAYANAGI Hajime
    Department of Physiology, Showa University School of Medicine
  • KANEKI Kiyomi
    Department of Physiology, Showa University School of Medicine
  • KITAMURA Atsuko
    Department of Physiology, Showa University School of Medicine
  • OKADA Mayumi
    Department of Anesthesiology, Showa University School of Medicine
  • TOKITA Erika
    Department of Kampo Medicine, Showa University School of Medicine Department of Otorhinolaryngology, Showa University School of Medicine
  • IWANAMI Hiroaki
    Department of Kampo Medicine, Showa University School of Medicine Department of Medicine, Division of Neurology, Showa University School of Medicine
  • HORIBE Yuzo
    Department of Kampo Medicine, Showa University School of Medicine Department of Medicine, Division of Neurology, Showa University School of Medicine
  • ISHINO Shogo
    Department of Physiology, Showa University School of Medicine Department of Kampo Medicine, Showa University School of Medicine
  • HISAMITSU Tadashi
    Department of Physiology, Showa University School of Medicine

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Other Title
  • モルヒネ誘発性便秘モデルマウスにおいて大建中湯はカハール介在細胞の減少を抑制する
  • モルヒネ ユウハツセイ ベンピ モデルマウス ニ オイテ ダイケンチュウ ユ ワ カハール カイザイ サイボウ ノ ゲンショウ オ ヨクセイ スル

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Daikenchuto （DKT） is a traditional herbal medicine （also referred to as Kampo medicine） which has been used to treat postoperative ileus, intestinal paralysis, stomachache, abdominal distention, constipation and diarrhea. The aim of the study was to clarify the effect of DKT on constipation induced by the chronic administration of morphine and its influence on the morphology and quantity of interstitial cells of Cajal （ICC）. 1） Male C57BL/6J mice were injected with morphine hydrochloride （10mg/kg） subcutaneously once a day for 10 days. DKT （30, 75, 150, 300, and 500 mg/kg） was administered 60 min before the injection of morphine. Only the administration of DKT （75mg/kg） inhibited the decrease in the volume of stool. 2） The pain threshold was measured using the tail flick test 30min after the injection of morphine. The administration of DKT did not influence the antinociceptive effect of morphine. 3） The expression of ICC was examined immunohistochemistrically. The number of ICC in the upper part of the small intestine and the colon were reduced by the chronic administration of morphine; however, the reductions were inhibited due to the administration of DKT （75mg/kg）. Because DKT did not prevent the antinociceptive effect of morphine, the effect of DKT is not mediated through blocking the opioid receptor. ICC is known to serve as a “pacemaker” which regulates contraction of the smooth muscle. Our results suggest that the reduction in the number of ICC was involved in morphine-induced constipation and the administration of DKT inhibited the decrease in the volume of stool. Administration of the optimal dose of DKT may prevent morphine-induced constipation.

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