Simultaneous Quantitative Analysis of HIV Protease Inhibitors in Human Plasma Using Core-Shell Column and Fast MRM Detection
-
- WATANABE Kyoko
- Life Sciences Mass Spectrometry, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne Global Application Development Center, Analytical & Measurement Instruments Division, Shimadzu Corporation
-
- KUEHN Eliane
- Life Sciences Mass Spectrometry, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne
-
- VARESIO Emmanuel
- Life Sciences Mass Spectrometry, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne
-
- HOPFGARTNER Gérard
- Life Sciences Mass Spectrometry, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne
Search this article
Abstract
To support therapeutic drug monitoring (TDM) a quantitative method for eight HIV protease inhibitors (PIs): nelfinavir, amprenavir, indinavir, saquinavir, atazanavir, darunavir, ritonavir and lopinavir in human plasma was developed. Separation of the analytes was achieved using core-shell column for UHPLC separation and mass spectrometric detection in fast multiple reaction monitoring (MRM) mode. Human plasma samples were collected on filter paper in the tube-based dried blood spot device and the analytes were extracted with methanol directly. Core-shell column and fast MRM allowed achieving an analysis time of six minutes. Using a plasma aliquot of 15 µL a dynamic linear range of 25-5000 ng/mL (ritonavir), 25-10000 ng/mL (nelfinavir and amprenavir) and 25-20000 ng/mL (indinavir, saquinavir, atazanavir, darunavir and lopinavir) could be achieved with precision and accuracies better than 15 %.
Journal
-
- CHROMATOGRAPHY
-
CHROMATOGRAPHY 36 (3), 127-131, 2015
The Society for Chromatographic Sciences
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390001205744795264
-
- NII Article ID
- 130005118193
-
- NII Book ID
- AA1137755X
-
- ISSN
- 13483315
- 13428284
-
- NDL BIB ID
- 027004313
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- Abstract License Flag
- Disallowed