Scanning and Transmision Electron Microscopic Studies on Human Term Placental Villi

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  • CHEN CHING-LANG
    Departnent of Obstetrics and Gynecology, lnntendo University School of Medicine.
  • SEIDA AKINORI
    Departnent of Obstetrics and Gynecology, lnntendo University School of Medicine.
  • OKUYAMA TERUAKI
    Departnent of Obstetrics and Gynecology, lnntendo University School of Medicine.
  • FURUYA HIROSHI
    Departnent of Obstetrics and Gynecology, lnntendo University School of Medicine.

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Other Title
  • 胎盤絨毛組織の透過型および走査型電子顕微鏡による所見について
  • タイバンジュウモウ ソシキ ノ トウカガタ オヨビ ソウサガタ デンシ ケンビキョウ ニ ヨル ショケン ニ ツイテ

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Abstract

The ultrastructure of the human term placental villi of normal pregnancy were studied by scanning and transmission electron microscope. The materials for observations were obtained at the time of cesarean section and normal vaginal delivery, fixed immediately with glutar-aldehyde and then dried with the critical point method in due course. Finally, ultrastructure of the tissues were observed by scanning electron microscope (Hitachi MSM-II and S-550) with a magnification of up to ×200-×20000, and by transmission electron microscope (Hitachi S-550) with a magnification of up to ×2160-×27000. The following results were obtained : I. Scanning electron microscope 1. Branches of the villi were observed to show a tree like fine structure as a whole. 2. On the surface of the placental villi turflike microvilli were seen without intertwining or sticking to each other. II. Transmission electron microscope 1. It was thought that many small vesicles close to the surface of the membrane of the syncytial cells indicate the occurrence of pinocytosis. 2. Many vesicles with granules in varying size were observed in the cytoplasm of the syncytial cells. 3. The mode of distribution of the microvilli on the surface of the villi were observed varying among the cells, someone few and someone absent. 4. The characteristics of the Langhans' cells were found to have fewer vesicles, lower total density and smaller RNP (ribonucleprotein) particles as compared with those of syncytial cells. 5. The cell membrane between the syncytium and the Langhans' cell was observed clearly. The desmosome on the cell membrane was sparsely. 6. The figure of transition to syncytial cells from Langhans' cells were unable to detected. 7. No definite cell membrane among the syncytium cells and a lot of syncytial knot in this layer were observed. 8. Thickness of the basement membrane, were measured to be about 300mu. On the basement membrane, it was found that more syncytium cells than the Langhans' cells are rested. 9. The stromal cell, bundles of collagen fiber and fine filament were seen in the layer of the stroma. 10. The endothelial cell, the basement membrane and the pericyte were observed in fetal capillary.

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