Studies on Northern Anthracnose of Red Clover, caused by Kabatiella caulivora (Kirch.) Karak : 2. Selection of resistant lines and clones

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  • アカクローバ茎割病に関する研究 : 第2報抵抗性系統および個体の選抜について
  • アカクローバ茎割病に関する研究-2-抵抗性系統および個体の選抜について
  • アカクローバ ケイカツビョウ ニ カンスル ケンキュウ 2 テイコウセイ ケイトウ オヨビ コタイ ノ センバツ ニ ツイテ

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The susceptibility of clones contained in the red clover cultivar (CV) to this disease was observed to distribute in a wide range. In the case of resistant CV, they contained a large number of resistant clones and a small number of susceptible ones. On the other hand, in that of susceptible CV, it was in an adverse relationship. To select resistant clones and lines, the following experiments were carried out. The plants were held for 48 hours at 19 ℃ in the moist chamber after inoculation, and removed to a green house under controlled conditions at 18 to 23 ℃ and were watered 4 to 5 times a day with an atomizer. The appropriate concentration of conidial suspension for selection of resistant clones was about 10^6 conidia per ml. The clones whose leaf petiolules only could be infected or those which showed no symptoms were selected as resistant clones. The susceptibility of the lines to this disease could easily be compared when artificially inoculated in the second trifoliate stage. The degree of susceptibility was expressed as the percentage of infected seedlings in all the inoculated seedlings. In this case, the appropriate concentration of conidial suspension in order to compare the susceptibility of the lines was 10^5 to 3×10^5 conidia per ml. In the screening test at the second trifoliate stage, the populations which showed the disease symptom earlier contained a larger number of susceptible clones and a smaller number of resistant clones than those which showed the symptom later.

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