Comparison of Detergent Method and Enzymatic Method for the Determination of Cell Wall Constituents of Feed Samples

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  • 細胞膜物質の定量における中性デタージエント法と酵素分析法との比較
  • 細胞膜物質の定量における中性デタージエント法と酵素分析法との比較〔英文〕
  • サイボウ マク ブッシツ ノ テイリョウ ニ オケル チュウセイ デタージエン

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Feed samples have been extracted by two digestion methods in order to prepare cell wall materials with low nitrogen contents. Two digestion methods involved extraction with neutral detergent and pronase. Furthermore the pretreatment method for removal of starch from feed samples was investigated with using α-amylase. Feed samples using in this experiment were as follows ; rice straw, orchardgrass, alfalfa, soybean meal, corn grain, wheat bran, defatted rice bran and corn silage. Pronase digestion showed higher rates of removal of total crude protein than that of neutral detergent digestion.

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