2,2'-Azobis (2-amidinopropane) dihydrochloride (AAPH)による小腸粘膜ホモジネートの脂質過酸化と膜結合性酵素の変性
書誌事項
- タイトル別名
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- Lipid Peroxidation and Membrane Bound Enzyme Denaturation of Homogenate of Small Intestinal Mucosa by 2,2'-Azobis (2-amidinopropane) dihydrochloride (AAPH)
- 2 2 Azobis 2 amidinopropane dihydrochloride AAPH ニ ヨル ショウチョウ ネンマク ホモジネート ノ シシツ カサンカ ト マク ケツゴウセイ コウソ ノ ヘンセイ
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To understand the effects of lipid peroxides on physiological function of small intestine, rat small intestinal mucosa was incubated with 2, 2'-azobis (2-amidinopropane) dihydrochloride (AAPH), a free radical generator in aqueous phase, and changes in the level of lipid peroxidation, protein content, and enzymatic activities of maltase, lactate dehydrogenase (LDH) and mitochondrial dehydrogenase were analyzed. We also examined preventive effects of α-tocopherol and L-ascorbic acid against such changes. Incubation of small intestinal mucosa with AAPH resulted in the increase of thiobarbituric acid reactive substances (TBARS). Similar treatment induced reduction of the maltase activity. Addition of α-tocopherol and L-ascorbic acid prevented the increase of TBARS induced with AAPH, although the inhibitory effect on maltase level did not change unless 500 μg/mg protein of L-ascorbic acid was added. Likewise AAPH lowered the level of LDH and mitochondrial dehydrogenase activities, which was not influenced by α-tocopherol. These results demonstrate that AAPH treatment causes cellular damage, indicating that both lipids and proteins are attacked by free radicals. Further investigation is necessary to clear preventive effects of antioxidative vitamins against change in proteins and cellular injury caused with AAPH in small intestine.
収録刊行物
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- ビタミン
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ビタミン 73 (5-6), 303-308, 1999
公益社団法人 日本ビタミン学会
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詳細情報 詳細情報について
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- CRID
- 1390001205760570240
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- NII論文ID
- 110002843842
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- NII書誌ID
- AN00207833
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- ISSN
- 2424080X
- 0006386X
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- NDL書誌ID
- 4765483
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可