書誌事項
- タイトル別名
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- Investigation of the Pathways of Biosynthesis and Metabolism of NAD in Euglena
- ユーグレナにおけるNADの生合成と代謝の経路に関する検討
- ユーグレナ ニ オケル NAD ノ セイゴウセイ ト タイシャ ノ ケイロ ニ カンスル ケントウ
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説明
In order to obtain information on NAD biosynthetic and metabolic pathways of Euglena, we investigated the effects of NAD precursors on growth and various enzyme activities in Euglena gracilis Z. Among the NAD precursors, dihydroxyacetone phosphate had no effect on growth of Euglena gracilis Z cultured heterotrophically at a concentration of 5 mM, while NAD content increased about 1.3 fold. We found that quinolinic acid was biosynthesized from aspartic acid (Asp) and dihydroxyacetone phosphate in Euglena gracilis Z, suggesting that NAD may be biosynthesized via Asp-pathway. Among enzymes related to NAD biosynthesis and metabolism, nicotinamidase, nicotinate phosphoribosyltransferase, nicotinic acid mononucleotide adenyltransferase, and NAD synthetase activities were all found in Euglena gracilis Z. In contrast, activities of quinolinate phosphoribosyltransferase, nicotinamide mononucleotide adenyltransferase, nicotinamide phosphoribosyltransferase, nicotinate methyltransferase, and nicotinamide methyltransferase were barely or little detected. These results suggest that NAD may be biosynthesized in a salvage pathway via nicotinic acid. Nicotinate phosphoribosyltransferase was inhibited by nicotinic acid mononucleotide and nicotinic acid adenine dinucleotide, whereas nicotinamidase by nicotinamide mononucleotide, NAD, and N^1-methylnicotinamide. We suggest that Euglena had its own unique regulatory mechanism in the biosynthesis and metabolism of NAD.
収録刊行物
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- ビタミン
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ビタミン 75 (1), 15-21, 2001
公益社団法人 日本ビタミン学会
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詳細情報 詳細情報について
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- CRID
- 1390001205783496832
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- NII論文ID
- 110002883430
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- NII書誌ID
- AN00207833
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- ISSN
- 2424080X
- 0006386X
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- NDL書誌ID
- 5648817
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
