Incorporation of ^<14>C-Pantothenic Acid into an Intermediate of CoA Biosynthesis in Rat Liver
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- KUWAGATA Masaomi
- Department of Pediatrics and Biochemistry, Kyoto Prefectual University of Medicine
Bibliographic Information
- Other Title
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- ^<14>C-パントテン酸のシロネズミ肝CoA生合成中間体へのとりこみ
- 14C-パントテンサン ノ シロネズミ ギモ CoA セイゴウセイ チュウカンタイ エ ノ トリコミ
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Description
In order to clarify a metabolic role of pantothenic acid (PaA) derivatives accumulated in rat liver, in the experiments with ^<14>C-pantothenic acid, incorporation of the isotope into the derivatives was determined. The PaA derivatives were extracted from the liver of PaA-deficient rat after administration of ^<14>C-PaA for eight days, then applied on DEAE-cellulose column chromatography. Three radiocative fractions, peak A, B and C were obtained and also detected by microbiological assay with L. arabinosus. It was found that peak A was identified with paA, peak B with 4'-phosphopantetheine and peak C with CoA, respectively, by means of paper chromatography. The ratio of radioactivity per pantothenic acid of the three compounds were 2 : 1 : 1. In the additional labelling experiment, however, the specific radioactivity of peak B (4'-phosphopantetheine) was 2.5 times as much as that of peak C (CoA). This suggests that 4'-phosphopantetheine existed in rat liver acts as a precursor of CoA biosynthesis, but not a decomposition product of CoA.
Journal
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- VITAMINS
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VITAMINS 43 (2), 78-86, 1971
THE VITAMIN SOCIETY OF JAPAN
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Details 詳細情報について
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- CRID
- 1390001205808916096
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- NII Article ID
- 110002850284
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- NII Book ID
- AN00207833
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- ISSN
- 2424080X
- 0006386X
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- NDL BIB ID
- 8424928
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- CiNii Articles
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- Abstract License Flag
- Disallowed