TRANSPORT OF VITAMIN B_6 IN HUMAN ERYTHROCYTES : (IV) EFFECTS OF INCUBATION TEMPERATURE, GLYOCLYSIS INHIBITOR AND VITAMIN B_6 ANALOGUES ON THE TRANSPORT OF VITAMIN B_6 INTO HUMAN ERYTHROCYTES

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  • YAMADA Kozo
    Department of Internal Medicine, School of Medicine, University of Nagoya
  • TSUJI Minoru
    Department of Internal Medicine, School of Medicine, University of Nagoya

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Other Title
  • ビタミンB_6の血球内移行にかんする研究 : (IV)ビタミンB_6各型の血球内移行にたいする保温温度, 解糖阻害剤およびビタミンB_6拮抗物質の影響
  • ビタミンB6の血球内移行にかんする研究-4-ビタミンB6各型の血球内移行にたいする保温温度,解糖阻害剤およびビタミンB6拮抗物質の影響
  • ビタミン B6 ノ ケッキュウ ナイ イコウ ニ カンスル ケンキュウ 4 ビタミン B6 カクカタ ノ ケッキュウ ナイ イコウ ニ タイスル ホオン オンド , カイトウ ソガイザイ オヨビ ビタミン B6 キッコウ ブッシツ ノ エイキョウ

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Abstract

Studies were made on the effects of incubation temperature, glycolysis inhibitor and vitamin B_6 analogues on the transport of B_6 into human erythrocytes. The transported amounts of pyridoxine and pyridoxamine into the cells diminished in the low temperature, but pyridoxal did not. Potassium fluoride inhibited the transport of pyridoxine, pyridoxamine and pyridoxal phosphate into the cells, but did not the transport of pyridoxal. Furthermore, pyridoxal phosphate concentrations in the cells after incubation with free forms of B_6 were decreased by the addition of potassium fluoride. Deoxypyridoxine inhibited competitively the transport of pyridoxine and pyridoxamine into the cells, but did not the transport of pyridoxal and pyridoxal phosphate. Pyridoxal phosphate concentrations in the cells after incubation with free forms of B_6 were decreased by the addition of deoxypyridoxine. Deoxypyridoxine phosphate did not inhibited the transport of various forms of B_6.

Journal

  • VITAMINS

    VITAMINS 37 (5), 472-478, 1968

    THE VITAMIN SOCIETY OF JAPAN

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