Separation and Identification of the B_6-Dependent Fatty Acid Synthetase from Epidermal Mitochondria

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  • SAKAMOTO Nobuo
    Department of Dermatology, School of Medicine, Chiba University
  • FUJITA Masaru
    Department of Dermatology, School of Medicine, Chiba University
  • ASO Kazuo
    Department of Dermatology, School of Medicine, Chiba University

Bibliographic Information

Other Title
  • 表皮ミトコンドリアのビタミンB_6依存性脂肪酸合成酵素について
  • ヒョウヒ ミトコンドリア ノ ビタミン B6 イゾンセイ シボウサン ゴウセイ コウソ ニ ツイテ

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Abstract

The incorporation of acetyl CoA into fatty acid by epidermal mitochondria was confirmed. Elongation of short chain fatty acids with one C_2 unit-acetyl-CoA was a major reaction. The dialyzate of the freezed and thawed epidermal mitochondria lost its activity, which was recovered completly by adding 0.4μm of pyridoxal phosphate. The enzymic activity was purified to 133 fold the potency after DEAE-cellulose column treatment. ATP, NADPH and NADH were required as cofactors.

Journal

  • VITAMINS

    VITAMINS 42 (5), 280-287, 1970

    THE VITAMIN SOCIETY OF JAPAN

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