Determination of Urinary Methylmalonic Acid by Isotachophoresis

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  • KAWATA Tetsunori
    Laboratory of Nutrition and Food Chemistry, Department of Agricultural Chemistry, Tokyo University of Agriculture
  • INUI Takayuki
    Laboratory of Nutrition and Food Chemistry, Department of Agricultural Chemistry, Tokyo University of Agriculture
  • MAEKAWA Akio
    Laboratory of Nutrition and Food Chemistry, Department of Agricultural Chemistry, Tokyo University of Agriculture
  • SUZUKI Takao
    Laboratory of Nutrition and Food Chemistry, Department of Agricultural Chemistry, Tokyo University of Agriculture

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Other Title
  • 細管式等速電気泳動法によるシロネズミ尿中メチルマロン酸の測定
  • サイカンシキ トウソク デンキ エイドウホウ ニヨル シロネズミ ニョウチュウ

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Description

The determination of methylmalonic acid (MMA) in rat urine were studied by use of isotachophoresis. This method is based on measurement of potential unit value of each organic acid. The operational systems for determination of MMA by isotachophoresis were devised. The leading electrolyte at pH of 3.20 using 0.01M HCl and β-alanine was selected as the most suitable. Moreover, the counterflow technique makes it possible to obtain a complete separation of MMA in organic acid mixture and rat urine. The recovery of MMA added to rat urine reached to 94~101%, when the urine was pretreated by Dowex AG 3×4 column. This method for determination of MMA in rat urine by isotachophoresis is simple, rapid and quantitative.

Journal

  • VITAMINS

    VITAMINS 54 (1), 11-15, 1980

    THE VITAMIN SOCIETY OF JAPAN

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