Slow-Binding Inhibition and Slow, Tight-Binding Inhibition of Aspartate Aminotransferase by Hydrazinosuccinate
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- YAMADA Ryo-hei
- Department of Biochemistry, Kyoto Prefectural University of Medicine
Bibliographic Information
- Other Title
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- ヒドラジノコハク酸によるアスパラギン酸アミノ基転移酵素の Slow-binding inhibition 及び Slow, tight-binding inhibition
- ヒドラジノコハク類によるアスパラギン酸アミノ基転移酵素のSlow-binding inhibition及びSlow,tight-binding inhibition
- ヒドラジノコハクルイ ニ ヨル アスパラギンサン アミノキ テンイ コウソ ノ
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Abstract
Administration of isoniazid to mice induced a remarkable inhibition of liver cytosolic aspartate aminotransferase. Experimental findings indicated that the inhibitor was not isoniazid itself but probably its metabolite. DL-Hydrazinosuccinate, a model compound of the metabolite, was synthesized since the metabolite was not successfully isolated. The compound was found to be even more inhibitory. The inhibition by the compound proceeded time-dependently and was not readily released. D- and L-Hydrazinosuccinate were synthesized, respectively, for further analyses of the inhibition mechanisms. D-Hydrazinosuccinate was found to behave as a slow-binding inhibitor via a single-step reaction mechanism and gave Ki of approx. 3 nM. L-Hydrazinosuccinate was a more powerful inhibitor with Ki of approx. 0.2 nM, and was found to be a slow, tight-binding inhibitor and interact with the enzyme through consecutive reversible steps. Administration of L-hydrazinosuccinate to mice produced in vivo a potent and long lasting inhibition of cytosolic aspartate aminotransferase in the liver and kidney in a relatively specific manner. The administration of this compound also caused a remarkable accumulation of citrulline in the liver and plasma, which was explained as a result of both specific and non-specific effects of the inhibitor.
Journal
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- VITAMINS
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VITAMINS 62 (3), 117-127, 1988
THE VITAMIN SOCIETY OF JAPAN
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Keywords
Details 詳細情報について
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- CRID
- 1390001205832675840
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- NII Article ID
- 110002847540
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- NII Book ID
- AN00207833
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- ISSN
- 2424080X
- 0006386X
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- NDL BIB ID
- 3175383
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- CiNii Articles
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- Abstract License Flag
- Disallowed