書誌事項
- タイトル別名
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- 30pm1-A-5 Single-Particle Co-Culture System Using Collagen Janus Microparticle
抄録
This paper describes anisotropic spherical collagen hydrogel microparticles (〜100 μm in diameter) for single-particle co-culture system. We fabricated a quantity of uniform-sized collagen microparticles easily by ejecting micro-droplets containing collagen solution and sodium alginate into calcium chloride solution under high centrifugal gravity by using centrifuge-based microfluidic device composed of theta capillary, capillary holder, and microtube. We successfully demonstrated in encapsulating differently-labeled NIH/3T3 cells in each hemisphere at high viability by using our collagen Janus microparticles. We believe that our collagen Janus microparticles could be available to control differentiation of stem cells in a heterogeneous microenvironment at single-particles and to co-culture various cells in different ECMs with massive and quantitative analyses.
収録刊行物
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- マイクロ・ナノ工学シンポジウム
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マイクロ・ナノ工学シンポジウム 2015.7 (0), _30pm1-A-5-_30pm1-A-5, 2015
一般社団法人 日本機械学会
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詳細情報 詳細情報について
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- CRID
- 1390001205903052672
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- NII論文ID
- 110010046065
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- ISSN
- 24329495
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可