Conparison of the N-Terminal Sequences of Three Glutaminases from <I>Bacillus subtilis</I>

DOI

Bibliographic Information

Other Title
  • 枯草菌の生産するグルタミナーゼのN末端配列の比較
  • Conparison of the N-Terminal Sequences of Three Glutaminases from Bacillus subtilis

Abstract

Three glutaminases [EC 3. 5. 1. 2] from different Bacillus subtilis strains which were effective in high salt concentration environment such as miso were purified by ion-exchange, hydrophobic and gel filtration chromatographies. The enzymes were composed of two subunits with different mass, and they were estimated to be about 42kDa and 22 kDa by SDS-PAGE. After electro-blotted onto PVDF membrane, N-terminal sequences were revealed by automatic sequencing that they were homologous with that of γ-glutamyltranspeptidase [EC 2. 3. 2. 2].

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Details 詳細情報について

  • CRID
    1390001206102393344
  • NII Article ID
    130004104888
  • DOI
    10.6013/jbrewsocjapan1988.89.570
  • COI
    1:CAS:528:DyaK2cXlslertL0%3D
  • ISSN
    21864012
    09147314
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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