機能性サイトカイン変異体の創製による次世代バイオ医薬品の開発

DOI Web Site Web Site PubMed 参考文献13件 オープンアクセス
  • 鎌田 春彦
    国立研究開発法人医薬基盤・健康・栄養研究所バイオ創薬プロジェクト

書誌事項

タイトル別名
  • Development of New Biologics through Creation of a Functional Cytokine Mutant
  • Symposium Review 機能性サイトカイン変異体の創製による次世代バイオ医薬品の開発
  • Symposium Review キノウセイ サイトカイン ヘンイタイ ノ ソウセイ ニ ヨル ジセダイ バイオ イヤクヒン ノ カイハツ

この論文をさがす

抄録

  The clinical use of cytokines is fairly limited because of their characteristics of having significant bioactivity and low stability, although some are useful biopharmaceuticals, such as interferon. Cytokines, which are secreted from various immune cells, show many kinds of bioactivities including unexpected activities; thus it would be desirable to regulate cytokine activity. Recently, we have developed a new drug delivery system (DDS) to create structural mutant cytokines using a phage display system. This system can produce functional mutant proteins that can bind their objective targets specifically. In this study, tumor necrosis factor (TNF) was used as a model cytokine to create agonist and antagonist activities against two TNF receptors TNFR1 and TNFR2, respectively. We created a phage library expressing mutant TNF, where the amino acids in the binding interface between TNF and TNF receptors were alternately exchanged. Affinity panning was performed at the optimum condition and the bioactivities of these mutant TNFs were analyzed to obtain the objective agonists or antagonists. The pharmacological activity and toxicity of these engineered TNF mutants could indicate their potential use as novel biopharmaceutical agents.<br>

収録刊行物

  • 薬学雑誌

    薬学雑誌 135 (7), 843-849, 2015-07-01

    公益社団法人 日本薬学会

参考文献 (13)*注記

もっと見る

関連プロジェクト

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ