Estimates in several industrial yeast strains ploidy by flow cytometer with rapamycin.
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- MIKI Takeo
- <i>Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi</i>
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- MURAMATSU Noboru
- <i>Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi</i>
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- SOEKAWA Kazuhiro
- <i>Hyashi Winery Co, Ltd.</i>
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- IKARI Shinji
- <i>Hyashi Winery Co, Ltd.</i>
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- HAYASHI Mikio
- <i>Hyashi Winery Co, Ltd.</i>
Bibliographic Information
- Other Title
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- ラパマイシンを用いた実用酵母株のフローサイトメーターによる倍数性の推定
- ラパマイシン オ モチイタ ジツヨウ コウボカブ ノ フローサイトメーター ニ ヨル バイスウセイ ノ スイテイ
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Abstract
The present work investigated ploidy measurement methods by flow cytometer (FCM) in industrial yeast treated with rapamycin. Four industrial yeast strains such as Saccharomyces cerevisiae OC-2, W-3, KN-35 and KN94, growing in a logarithmic phase with rapamycin, had their ploidy analyzed by FCM. It was apparent that, compared with the case of rapamycin, the untreated peak indicating the DNA amount of the yeast cells as determined by FCM showed increased accuracy over conventional ploidy yeast measurements. Comparison with S. cerevisiae OC-2 and W-3 as diploids estimated the ploidy of industrial yeasts KN-94 strain to be aneuploid or triploid, and the KN-35 strain to be aneuploid or diploid.
Journal
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- JOURNAL OF THE BREWING SOCIETY OF JAPAN
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JOURNAL OF THE BREWING SOCIETY OF JAPAN 110 (2), 105-109, 2015
Brewing Society of Japan
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Details
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- CRID
- 1390001206127249024
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- NII Article ID
- 130006694362
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- NII Book ID
- AN10034389
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- ISSN
- 21864012
- 09147314
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- NDL BIB ID
- 026154710
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed