生体試料の前処理法として水系ゲルろ過カラムを接続したクロマトグラフィー

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タイトル別名
  • Coupled Column Chromatography Using Hydrophilic Gel Permeation Column to Clean up Biological Fluid
  • セイタイ シリョウ ノ マエショリホウ ト シテ スイケイ ゲル ロカ カラム

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抄録

A simplified coupled column technique for the simultaneous determination of cilostamide and its metabolites in biological fluid by high performance liquid chromatography (HPLC) was developed. In this method, the plasma and urine containing an intact drug and its metabolites are injected into HPLC system directly. The effluent from the primary exclusion column is passed continuously through a four-port valve to waste. When monitoring of the elution of the biopolymers by the refractive index (RI) detector is completed, the valve diverts the metabolites and intact drug concentrated on the hydrophilic gel permeation column to the secondary column for separation. Their separation is achieved by using a gradient elution to elute the sample from the secondary column, and their detection is performed by a ultraviolet (UV) detector and a fluorescence (FL) detector. The detection limit is about 3 ng with the injection volume used in this method, and the coefficient of variation is less than 1% in retention time and less than 3% in the peak height.

収録刊行物

  • 薬学雑誌

    薬学雑誌 103 (11), 1174-1179, 1983

    公益社団法人 日本薬学会

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