Simultaneous Determination of Ropivacaine, Bupivacaine and Dexamethasone in Biodegradable PLGA Microspheres by High Performance Liquid Chromatography
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- ZHOU Zhifeng
- School of Public Health and Tropical Medicine, Southern Medical University
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- YE Jufeng
- School of Public Health and Tropical Medicine, Southern Medical University
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- CHEN Lingyun
- School of Public Health and Tropical Medicine, Southern Medical University
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- MA Ande
- School of Public Health and Tropical Medicine, Southern Medical University
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- ZOU Fei
- School of Public Health and Tropical Medicine, Southern Medical University
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A simple and rapid high-performance liquid chromatography method coupled with UV detector was developed and validated for the simultaneous determination of ropivacaine, bupivacaine and dexamethasone in biodegradable poly(lactic-co-glycolic acid) (PLGA) microspheres within 11 min. Chromatographic separation was performed on a XDB-C18 column using a mobile phase comprised of acetonitrile-NaH2PO4 buffer (pH 3.5, 30 mM) (30:70, v/v) with a flow rate gradient program. The method was in good linearity (r>0.999) over the range of 0.025-40.0 μg/ml for ropivacaine and bupivacaine, and 0.05-40 μg/ml for dexamethasone. The method was proved to be precise with intra- and inter-day precision less than 3.0% and 6.0% for all drugs and accurate with intra- and inter-day accuracy between -8.0% to 4.5% and between -5.0% to 5.5% for all drugs. The assay was rapid, simple and easy to apply. Therefore, it was very suitable for routine determination and quality control of ropivacaine, bupivacaine and dexamethasone in PLGA microspheres.<br>
収録刊行物
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- 薬学雑誌
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薬学雑誌 130 (8), 1061-1068, 2010-08-01
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001206128233216
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- NII論文ID
- 130000308772
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- NII書誌ID
- AN00284903
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- ISSN
- 13475231
- 00316903
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- NDL書誌ID
- 10794781
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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