Administration of Antigen to Digestive Tract and Formation of Antibodies in Mice

KURATA MUNETSUGU, KOYAMA IWAO, KUWAE TOYOYASU

doi:10.1248/yakushi1947.100.10_1004

Class of antibodies formed in mice by the oral, rectal and intraperitoneal (i.p.) administration with egg albumin (EA) was investigated. IgM antibody to EA was formed in the following cases ; everyday oral administration of 3.0 mg of EA, everyday rectal administration of 0.4 mg of antigen and i.p. injection at intervals of 3 days of 1.0 mg of antigen, and detected at 8th, 7th and 6th days, respectively, after the first immunization. IgG antibody appeared on the next day of IgM antibody detection. Thus IgM and IgG antibodies were produced even by the rectal administration of antigen, and it is suggested that antigen is absorbed from the digestive system as a molecule (macromolecule) enough to show immunogenicity. The oral and rectal administration showed similar effect as an administration route with regard to an increase in the blood IgA level and non-induction of IgE antibody to EA. IgE antibody was induced by the i.p. injection with not only native EA but also EA which was partially digested with pepsin and trypsin in vitro, and IgE antibody titer to native EA was higher at 10μg than 100μg of the dose of native antigen. These findings suggest that the non-induction of IgE antibody by orally administration of EA did not depend upon that protein becomes smaller molecules by proteases.

Administration of Antigen to Digestive Tract and Formation of Antibodies in Mice

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